Current manufacturing procedures for vaccines, biologics, and cell therapeutics routinely employ two-dimensional (2D) culture systems such as roller bottles or cell cubes/factories for expansion of cells. For anchorage dependent cell types, microcarriers offer a three-dimensional (3D) surface with a larger surface area to volume ratio. This supports dense systems at a larger scale with a relatively small footprint in the manufacturing suite.
Pall SoloHill solid core microcarriers are designed to meet the challenges faced by today’s cell and tissue culture researchers. Whether moving an existing process out of a 2D system or scaling up a new process, SoloHill microcarriers offer solutions to meet your needs
Pall SoloHill microcarriers are designed to support cell growth at a variety of scales: spinner flasks, microbioreactors , small to large scale stirred tank bioreactors , rocking platforms and microgravity bioreactors.
When cells are expanded for use in patient care or in vivo assays it is important to manage particulate levels in the final cell suspension. Hillex® CT microcarriers are designed specifically for these sensitive applications. The newest microcarrier in the SoloHill microcarrier line, Hillex CT microcarriers have the lowest particulate content of any microcarrier available today. They are the ideal substrate for expansion of stem cells for research and cellular therapy applications.
Hillex CT microcarriers may be custom ordered in ready-to-transfer custom packaging. Contact us to discuss gamma irradiation and single-use bioprocessing bags for easy integration into your single-use or traditional bioreactors.
Click Here , to learn more about Hillex CT and other Animal Product Free microcarriers.
Microcarriers can significantly reduce cell culture production costs. This reduction becomes clear when traditional cell culture methods are compared to microcarriers in bioreactors. In this economic model, cell cubes, roller bottles and SoloHill microcarriers in bioreactors are compared over a five year campaign. Total cost for each methodology is based on 2,000,000 cm² of surface area and 24 runs per year. Serum and media costs are estimated at $25 per liter. This model includes all capital equipment & depreciation, material expenses, serum + media, space requirements and all labor costs. SoloHill microcarriers offer a $3M savings over 5 years.
The most common method of harvesting cells from any microcarrier involves the use of proteolytic enzymes such as trypsin or collagenase. Because the cell filipodia dissolve more quickly from a smooth, nonporous bead than other types of substrates, cells harvested from SoloHill microcarriers are more robust and viable for the next stage of the process. Internal and customer testing shows that whole infected cells exhibit high viability post-harvest from SoloHill microcarriers.
SoloHill microcarriers are especially suited to scale up operations using strip and replate methods. High viability supports success in three-step scale-up processes. Cells may be expanded in a small bioreactor, repopulated into a five liter system for final seeding into a twenty five liter system. SoloHill microcarriers ensure minimal losses with each transfer and allow large quantities of cells to be generated prior to infection.
SoloHill microcarriers also support bead-to-bead transfer methods which allow expansion without the use of dissolution enzymes. In this method, unpopulated carriers are introduced to an existing culture. In the proper conditions, cells migrate from established carriers to new carriers for further expansion.
A variety of veterinary and human (FDA-licensed) products are currently produced using SoloHill microcarriers. This includes diverse products such as lytic and nonlytic viruses and expressed proteins from stable cell lines and transient transfected cells. SoloHill microcarriers are FDA-registered with six Drug Master File numbers.
Microcarriers are tiny spheres normally in the range of 90-300 μm in diameter. They are designed with a surface chemistry which allows for attachment and growth of anchorage dependent cell lines. Densities in the range of 1.02-1.10 g/cm³ allow them to be maintained in suspension with gentle stirring. The solid polystyrene core of the SoloHill Microcarrier prevents absorption of serum, cell culture products and dissociation enzymes.
Cells readily attach to a polystyrene surface under low stress conditions, but adhesion is less efficient in the high shear stress environment found in suspension cultures or bioreactors. For these conditions SoloHill microcarriers are available with a variety of coatings such as glass, collagen (gelatin), recombinant proteins or chemical treatments to enhance cell attachment. The ideal substrate can ensures successful cell yields for many different cell lines. In addition, SoloHill microcarriers are extremely durable and non-fragmenting for long expressed-product runs and during cell separation.
Contact Us for guidance on determining the best microcarrier for your application.