TEST #11 Why Use Tangential Flow Filtration

Efficient and Cost Effective

  • TFF is easy to set up and use – Simply connect the TFF device to a pump and pressure gauge(s) with tubing and fittings, add your sample to the reservoir and you’re ready to go.
  • TFF is fast and efficient – It is easier to set up and much faster than dialysis. You can achieve higher concentrations in less time than with centrifugal devices or stirred cells.
  • Perform two steps with one system – You can concentrate and diafilter a sample on the same system, saving time and avoiding product loss.
  • TFF can be scaled-up or scaled-down – Materials of construction and cassette path length allow conditions established during pilot-scale trials to be applied to process-scale applications. TFF devices are available that can process sample volumes as small as 10 mL or as large as thousands of liters.
  • TFF is economical – TFF devices and cassettes can be cleaned and reused, or disposed of after single use. 간단한 완전성 테스트를 실시하여 멤브레인과 실(seal)의 손상 여부를 확인할 수 있습니다.

Tangential Flow Filtration 어플리케이션

  • Concentrate and desalt proteins and peptides
  • Concentrate and desalt nucleic acids
  • Recover and purify antibodies or recombinant proteins from cell culture media 
  • Recover and purify plasmid DNA from cell lysates or chromosomal DNA from whole blood
  • Fractionate dilute protein mixtures
  • Clarify cell lysates or tissue homogenates.

Pall Preferred 온라인 연구실 커뮤니티
  • Depyrogenate (remove endotoxin from) water, buffers, and media solutions
  • Prepare samples prior to column chromatography
  • Harvest cells
  • Recover or remove viruses